Development of stress tolerant Saccharomyces cerevisiae strains by metabolic engineering: New aspects from cell flocculation and zinc supplementation
Cheng Cheng, Mingming Zhang, Chuang Xue, Fengwu Bai, Xinqing Zhao

Highlights

・Development of tailored yeast strain for cellulosic ethanol production is proposed.
・Impact of cell flocculation on yeast stress tolerance was summarized.
・Effect of zinc supplementation on yeast stress tolerance was reviewed.

Identification of a putative FR901469 biosynthesis gene cluster in fungal sp. No. 11243 and enhancement of the productivity by overexpressing the transcription factor gene frbF
Makoto Matsui, Tatsuya Yokoyama, Kaoru Nemoto, Toshitaka Kumagai, Goro Terai, Koichi Tamano, Masayuki Machida, Takashi Shibata

Role of the Tyr270 residue in 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase from Mesorhizobium loti
Jun Kobayashi, Hiromi Yoshida, Toshiharu Yagi, Shigehiro Kamitori, Hideyuki Hayashi, Kimihiko Mizutani, Nobuyuki Takahashi, Bunzo Mikami

Eichhornia crassipes: Agro-waster for a novel thermostable laccase production by Pycnoporus sanguineus SYBC-L1
Zhixin Wang, Jiayang Liu, Yawei Ning, Xiangru Liao, Yingmin Jia

Highlights

・Eichhornia crassipes could be used as the sole substrate for laccase production by Pycnoporus sanguineus SYBC-L1 in SSF.
・A novel purified Lac-S exhibited a remarkable cold catalytic efficiency.
・Lac-S was highly thermostable with a temperature range of 40-70 °C.

Purification and characterization of d-allulose 3-epimerase derived from Arthrobacter globiformis M30, a GRAS microorganism
Akihide Yoshihara, Taro Kozakai, Tomoya Shintani, Ryo Matsutani, Kouhei Ohtani, Tetsuo Iida, Kazuya Akimitsu, Ken Izumori, Pushpa Kiran Gullapalli

¹³C-metabolic flux analysis for mevalonate-producing strain of Escherichia coli
Keisuke Wada, Yoshihiro Toya, Satomi Banno, Katsunori Yoshikawa, Fumio Matsuda, Hiroshi Shimizu

Highlights

・Mevalonate (MVA) production in Escherichia coli having mvaES from Enterococcus faecalis was evaluated.
・Metabolic fluxes of control and MVA-producing strains were revealed by 13C-labeling.
・There were no significant differences in the fluxes of the upstream of acetyl-CoA.
・Fluxes of TCA cycle and acetate formation decreased in the MVA-producing strain.
・Fluxes suggested NADPH for MVA synthesis was obtained from transhydrogenase reaction.

Promoter engineering of the Saccharomyces cerevisiae RIM15 gene for improvement of alcoholic fermentation rates under stress conditions
Daisuke Watanabe, Akie Kaneko, Yukiko Sugimoto, Shinsuke Ohnuki, Hiroshi Takagi, Yoshikazu Ohya

Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
Marie Okumura, Yoshiko Fujitani, Masahiko Maekawa, Jittima Charoenpanich, Hunja Murage, Kazuhide Kimbara, Nurettin Sahin, Akio Tani

Quality evaluation of green tea leaf cultured under artificial light condition using gas chromatography/mass spectrometry
Shunsuke Miyauchi, Tsutomu Yonetani, Takayuki Yuki, Ayako Tomio, Takeshi Bamba, Eiichiro Fukusaki

Influence of yeast and lactic acid bacterium on the constituent profile of soy sauce during fermentation
Risa Harada, Masanobu Yuzuki, Kotaro Ito, Kazuki Shiga, Takeshi Bamba, Eiichiro Fukusaki

Identification of 4-mercapto-4-methylpentan-2-one as the characteristic aroma of sake made from low-glutelin rice
Sachiko Iizuka-Furukawa, Atsuko Isogai, Kazutaka Kusaka, Tsutomu Fujii, Yoshinori Wakai

Microbial responses to various process disturbances in a continuous hydrogen reactor fed with galactose
Gopalakrishnan Kumar, Jeong-Hoon Park, Periyasamy Sivagurunathan, Sang-Hoon Lee, Hee-Deung Park, Sang-Hyoun Kim

Highlights

・H2 yield over 2 mol/mol galactose was achieved.
・Butyrate/acetate ratio is correlated with microbial community for first time.
・Clostridia and Bacilli were ecological competition relationship on H2 fermentation.

Simultaneous removal of carbon and nitrogen by mycelial pellets of a heterotrophic nitrifying fungus-Penicillium sp. L1
Yuxiang Liu, Tingting Hu, Jing Zhao, Yongkang Lv, Ruipeng Ren

Highlights

・A heterotrophic nitrifying fungus isolated.
・Mycelial pellets formed in liquid medium.
・Much higher tolerance to acid condition than common microorganism.
・The 48-h pellet inoculum better than spore suspension.
・Simultaneous removal of carbon and nitrogen.

Exploring high-affinity binding properties of octamer peptides by principal component analysis of tetramer peptides
Akiko Kume, Shun Kawai, Ryuji Kato, Shinmei Iwata, Kazunori Shimizu, Hiroyuki Honda

Enhanced cyanophycin production by Escherichia coli overexpressing the heterologous cphA gene from a deep sea metagenomic library
Jikun Du, Li Li, Shining Zhou

Extracellular protease derived from lactic acid bacteria stimulates the fermentative lactic acid production from the by-products of rice as a biomass refinery function
Masanori Watanabe, Charin Techapun, Ampin Kuntiya, Noppol Leksawasdi, Phisit Seesuriyachan, Thanongsak Chaiyaso, Shinji Takenaka, Isamu Maeda, Masahiro Koyama, Kozo Nakamura

Simultaneous engagement of mechanical stretching and surface pattern promotes cardiomyogenic differentiation of human mesenchymal stem cells
Seo Rin Gu, Yun Gyeong Kang, Ji Won Shin, Jung-Woog Shin

Disruption of myoblast alignment by highly motile rhabdomyosarcoma cell in tissue structure
Menglu Li, Eiji Nagamori, Masahiro Kino-oka

Development and evaluation of a removable tissue-engineered muscle with artificial tendons
Tomohiro Nakamura, Shunya Takagi, Takafumi Kamon, Ken-ichi Yamasaki, Toshia Fujisato

Development of a fluorescent protein-antibody Forster resonance energy transfer probe for the detection and imaging of osteocalcin
Chan-I Chung, Ryoji Makino, Yuki Ohmuro-Matsuyama, Hiroshi Ueda