Self-excising Cre/mutant lox marker recycling system for multiple gene integrations and consecutive gene deletions in Aspergillus oryzae
Silai Zhang, Akihiko Ban, Naoki Ebara, Osamu Mizutani, Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi

Characterization and functional expression of a rubber degradation gene of a Nocardia degrader from a rubber-processing factory
Dao Viet Linh, Nguyen Lan Huong, Michiro Tabata, Shunsuke Imai, Sou Iijima, Daisuke Kasai, To Kim Anh, Masao Fukuda

Enhanced in-cell folding of reversibly cationized transcription factor using amphipathic peptide
Midori Futami, Tomoki Nakano, Mayu Yasunaga, Masahiro Makihara, Takashi Asama, Yoshihisa Hagihara, Yoshihiro Nakajima, Junichiro Futami

Characterization of a novel DyP-type peroxidase from Streptomyces avermitilis
Kanako Sugawara, Yuriko Nishihashi, Tomomi Narioka, Toru Yoshida, Mifumi Morita, Yasushi Sugano

pH-gradient ion-exchange microbial cell chromatography as a simple method for microbial separation
Yoshiteru Aoi, Yuji Kaneko, Satoshi Tsuneda

Pyruvate dehydrogenase complex regulator (PdhR) gene deletion boosts glucose metabolism in Escherichia coli under oxygen-limited culture conditions
Soya Maeda, Kumiko Shimizu, Chie Kihira, Yuki Iwabu, Ryuichi Kato, Makoto Sugimoto, Satoru Fukiya, Masaru Wada, Atsushi Yokota

Highlights

・The effect of pdhR-deletion on glucose metabolism was evaluated in E. coli.
・pdhR deletion boosted glucose metabolism under oxygen-limited culture conditions.
・pdhR deletion enables energy-saving bioprocesses under less aeration in E. coli.
・PdhR appears to contribute to maintaining redox balance.

Expression of the alaE gene is positively regulated by the global regulator Lrp in response to intracellular accumulation of l-alanine in Escherichia coli
Kohei Ihara, Kazuki Sato, Hatsuhiro Hori, Yumiko Makino, Shuji Shigenobu, Tasuke Ando, Emiko Isogai, Hiroshi Yoneyama

Biosynthesis of eight-carbon volatiles from tomato and pepper pomaces by fungi: Trichoderma atroviride and Aspergillus sojae
Onur Guneser, Yonca Karagul Yuceer

Innovation Chinese rice wine brewing technology by bi-acidification to exclude rice soaking process
Xiao Lu Wei, Shuang Ping Liu, Jian Shen Yu, Yong Jian Yu, Sheng Hu Zhu, Zhi Lei Zhou, Jian Hu, Jian Mao

Ammonium conversion and its feedback effect on methane oxidation of Methylosinus sporium
Ruo He, Min Chen, Ruo-Chan Ma, Yao Su, Xuan Zhang

Effect of freeze-thaw process on physical properties, microbial activities and population structures of anaerobic sludge
Chantaraporn Phalakornkule, Sasikarn Nuchdang, Maneerat Khemkhao, Wuttichai Mhuantong, Sarunyou Wongwilaiwalin, Sithichoke Tangphatsornruang, Verawat Champreda, Jiraprat Kitsuwan, Savitri Vatanyoopaisarn

Highlights

・Freeze-thaw process reduced the sludge volume index from 16.4 to 4.0 mL/g.
・The freeze-thaw process enhanced acidogenic activity about 20%.
・Some acidogens were enriched in the solid fraction of the frozen-thawed sludge.
・The freeze-thaw process leveled down the abundance of some acetogens.
・No evidence for direct inhibition of methanogens due to the process was found.

Inhibition kinetics of nitritation and half-nitritation of old landfill leachate in a membrane bioreactor
Yun Li, Zhaozhao Wang, Jun Li, Jia Wei, Yanzhuo Zhang, Baihang Zhao

Pretreatment of lignocellulosic biomass by cattle rumen fluid for methane production: Bacterial flora and enzyme activity analysis
Yasunori Baba, Yu Matsuki, Yumi Mori, Yoshihisa Suyama, Chika Tada, Yasuhiro Fukuda, Masanori Saito, Yutaka Nakai

Effect of carbon source on nitrogen removal in anaerobic ammonium oxidation (anammox) process
Weiqiang Zhu, Peiyu Zhang, Huiyu Dong, Jin Li

Temperature dependence of nitrogen removal activity by anammox bacteria enriched at low temperatures
Giri Park, Masashi Takekawa, Satoshi Soda, Michihiko Ike, Kenji Furukawa

Cell compaction influences the regenerative potential of passaged bovine articular chondrocytes in an ex vivo cartilage defect model
Michael Schmutzer, Attila Aszodi

Rapid monitoring of RNA degradation activity in vivo for mammalian cells
Hidenori Tani, Hiroaki Sato, Masaki Torimura

Highlights

・We have developed a rapid fluorescence assay.
・Our new assay can rapidly quantify the RNA degradation rates in vivo.
・Our new assay eliminates time-consuming steps.

Spectrophotometric assay for sensitive detection of glycerol dehydratase activity using aldehyde dehydrogenase
Eul-Soo Park, Sunghoon Park, Jong-Shik Shin