Enzymatic synthesis of 2′-O-methylribonucleosides with a nucleoside hydrolase family enzyme from Lactobacillus buchneri LBK78
Yuuki Mitsukawa, Makoto Hibi, Narihiro Matsutani, Nobuyuki Horinouchi, Satomi Takahashi, Jun Ogawa

Identification of an acidic endo-polygalacturonase from Penicillium oxalicum CZ1028 and its broad use in major tropical and subtropical fruit juices production
Zhong Cheng, Dong Chen, Qingyan Wang, Liang Xian, Bo Lu, Yutuo Wei, Hongchi Tang, Zhilong Lu, Qixia Zhu, Yunlai Chen, Ribo Huang

Highlights

・Endo-polygalacturonase gene from Penicillium oxalicum was first time cloned.
・PoxaEnPG28A was overexpressed in Pichia pastoris with a high yield of 1828.7 U/mL.
・PoxaEnPG28A shared 72% identity with an endo-PG from Trichoderma harzianum.
・The recombinant PoxaEnPG28A was acidic and acid stable.
・PoxaEnPG28A showed remarkable catalytic activity on five kinds of fruit juices.

Improvement of operational stability of Ogataea minuta carbonyl reductase for chiral alcohol production
Kohsuke Honda, Mizuha Inoue, Tomohiro Ono, Kenji Okano, Yasumasa Dekishima, Hiroshi Kawabata

Evaluation of tyrosinase inhibitory and antioxidant activities of Angelica dahurica root extracts for four different probiotic bacteria fermentations
Guey-Horng Wang, Chih-Yu Chen, Teh-Hua Tsai, Ching-Kuo Chen, Chiu-Yu Cheng, Yi-Hsin Huang, Min-Chi Hsieh, Ying-Chien Chung

Highlights

・L. acidophilus-fermented ADR extract is suitable as a new cosmetic ingredient.
・Screening of bacterial species elevates the activity of fermented ADR extract.
・Fermentation enhances the activity of ADR extract due to production of new phenolics.

Reduction of FR900525 using an S-(2-aminoethyl) l-cysteine-resistant mutant
Shiho Shimizu, Ayako Futase, Tatsuya Yokoyama, Satoshi Ueda, Hiroyuki Honda

Biocatalyst-mediated production of 11,15-dihydroxy derivatives of androst-1,4-dien-3,17-dione
Yuqian Qiao, Yanbing Shen, Wei Huang, Yanping Wang, Jiajia Ren, Ting Xia, Min Wang

Injury and recovery of Escherichia coli ATCC25922 cells treated by high hydrostatic pressure at 400-600 MPa
Keitarou Kimura, Kazuya Morimatsu, Takashi Inaoka, Kazutaka Yamamoto

Breeding of a sake yeast mutant with enhanced ethyl caproate productivity in sake brewing using rice milled at a high polishing ratio
Toshinari Takahashi, Yusuke Ohara, Kazuo Sueno

Recycling of metals from pretreated waste printed circuit boards effectively in stirred tank reactor by a moderately thermophilic culture
Ming-Chen Xia, Ya-Ping Wang, Tang-Jian Peng, Li Shen, Run-Lan Yu, Yuan-Dong Liu, Miao Chen, Jiao-Kun Li, Xue-Ling Wu, Wei-Min Zeng

Highlights

・Moderately thermophiles were adapted in increasing PPCBs density causing improved tolerance and efficient copper recovery.
・Community structure was relatively simple during bioleaching at high PPCBs density.
・The treatment process combining shaking table separation with bioleaching would provide a feasible way for PCBs recycling.

Polyphosphate metabolism by purple non-sulfur bacteria and its possible application on photo-microbial fuel cell
Yen-Chun Lai, Chih-Ming Liang, Shu-Chuan Hsu, Ping-Heng Hsieh, Chun-Hsiung Hung

Influence of rice straw polyphenols on cellulase production by Trichoderma reesei
Wei Zheng, Qin Zheng, Yiyun Xue, Jiajun Hu, Min-Tian Gao

Highlights

・Phenolics from rice straw inhibited cellulase production by Trichoderma reesei.
・The release of phenolics affected cellulase production.
・Despite their low content, tannins greatly inhibited cellulase production.
・Ball milling affected the release of phenolics, especially phenolic acids.
・A tannin extraction before culturing improved cellulase production.

Fabrication of a fiber-type hepatic tissue by bottom-up method using multilayer spheroids
Tatsuya Okudaira, Ryohei Yabuta, Hiroshi Mizumoto, Toshihisa Kajiwara

Degradation of endothelial network in disordered tumor-containing cell sheet
Menglu Li, Masahiro Kino-oka

Evaluation of a novel system for analyzing hydrophilic blood metabolites
Shin Nishiumi, Keisuke Shima, Takeshi Azuma, Masaru Yoshida

Detection of antibodies against hepatitis B virus surface antigen and hepatitis C virus core antigen in plasma with a waveguide-mode sensor
Takenori Shimizu, Torahiko Tanaka, Shigeyuki Uno, Hiroki Ashiba, Makoto Fujimaki, Mutsuo Tanaka, Koichi Awazu, Makoto Makishima

Nanaomycin H: A new nanaomycin analog
Takuji Nakashima, Toru Kimura, Rei Miyano, Hirotaka Matsuo, Tomoyasu Hirose, Aoi Kimishima, Kenichi Nonaka, Masato Iwatsuki, Jun Nakanishi, Yoko Takahashi, Satoshi Omura