Calycosin promotes lifespan in Caenorhabditis elegans through insulin signaling pathway via daf-16, age-1 and daf-2
Lulu Lu, Xuan Zhao, Jianyong Zhang, Miao Li, Yonghao Qi, Lijun Zhou

Highlights

・Calycosin could function as an antioxidant to extend the lifespan of Caenorhabditis elegans.
・Calycosin requires insulin signaling to promote lifespan extension.
・Calycosin could enhance the nuclear translocation of DAF-16/FoXO instead of SKN-1/Nrf-2.
・The longevity effect of calycosin is likely not associated with the calorie restriction mechanism.

Further enhancement of FR901469 productivity by co-overexpression of cpcA, a cross-pathway control gene, and frbF in fungal sp. No. 11243
Makoto Matsui, Tatsuya Yokoyama, Kaoru Nemoto, Toshitaka Kumagai, Koichi Tamano, Masayuki Machida, Takashi Shibata

Nano-magnesium aided activity enhancement and biophysical characterization of a psychrophilic α-amylase immobilized on graphene oxide nanosupport
Nalok Dutta, Subrata Biswas, Malay Kumar Saha

Highlights

・MgNP supplemented amylase showed enhanced activity and stability at 8°C.
・MgNP-amylase immobilized on graphene showed stability at thermal extremes (8°C, 90°C).
・Enzymatic activity of GO-MgNP-amy retained after 12 repeated uses.
・Storage stability of GO-MgNP-amy at 4°C for more than 120 days.

First characterization of an archaeal amino acid racemase with broad substrate specificity from the hyperthermophile Pyrococcus horikoshii OT-3
Ryushi Kawakami, Haruhiko Sakuraba, Taketo Ohmori, Toshihisa Ohshima

Mutational analysis of cutinase-like enzyme, Cut190, based on the 3D docking structure with model compounds of polyethylene terephthalate
Takeshi Kawabata, Masayuki Oda, Fusako Kawai

In vitro preparation and assessment of radical reducing peptide from Octopus aegina using digestive proteases
Sekar Sudhakar, Rasool Abdul Nazeer

Highlights

・Octopus derived antioxidant peptide was found as Gly-Glu-Tyr.
・Tri peptide reduces DNA and lipid oxidative damage at in vitro.
・Antioxidant effect was probed to reduce ROS at intracellular level under oxidative stress.

Isolation of aquatic yeasts with the ability to neutralize acidic media, from an extremely acidic river near Japan's Kusatsu-Shirane Volcano
Daisuke Mitsuya, Takuya Hayashi, Yu Wang, Mami Tanaka, Masahiko Okai, Masami Ishida, Naoto Urano

Cell wall α-1,3-glucan prevents α-amylase adsorption onto fungal cell in submerged culture of Aspergillus oryzae
Silai Zhang, Hiroki Sato, Sakurako Ichinose, Mizuki Tanaka, Ken Miyazawa, Akira Yoshimi, Keietsu Abe, Takahiro Shintani, Katsuya Gomi

Construction of a novel d-lactate producing pathway from dihydroxyacetone phosphate of the Calvin cycle in cyanobacterium, Synechococcus elongatus PCC 7942
Yasutaka Hirokawa, Ryota Goto, Yoshitaka Umetani, Taizo Hanai

Highlights

・A novel lactate producing pathway was constructed in Synechococcus elongatus PCC 7942.
・Lactate was produced from DHAP in the Calvin cycle via methylglyoxal.
・The highest titer of lactate in this study reached 13.7 mM (1.23 g/L).

Quantitative evaluation of haze formation of koji and progression of internal haze by drying of koji during koji making
Kazunari Ito, Katsuya Gomi, Masahiro Kariyama, Tsuyoshi Miyake

Simultaneous chemical oxygen demand removal, methane production and heavy metal precipitation in the biological treatment of landfill leachate using acid mine drainage as sulfate resource
Yu-Long Li, Jin Wang, Zheng-Bo Yue, Wei Tao, Hai-Bin Yang, Yue-Fei Zhou, Tian-Hu Chen

Pilot scale production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) biopolymers with high molecular weight and elastomeric properties
Kai-Hee Huong, Mat Junoh Azuraini, Nursolehah Abdul Aziz, Al-Ashraf Abdullah Amirul

Highlights

・Reproducibility of P(3HB-co-4HB) with high molecular weight and elastomeric properties was studied.
・Sufficient oxygen supply during fermentation was found important in retaining the novel characteristics of the copolymer.
・KLa-based scale-up is important factor in retaining the copolymer quantity and quality in pilot scale.
・This work is beneficial to bioprocess research and manufacturing industry of polyhydroxyalkanoates.

Regioselective production of sulfated polyphenols using human cytosolic sulfotransferase-expressing Escherichia coli cells
Takehiko Shimohira, Katsuhisa Kurogi, Takuyu Hashiguchi, Ming-Cheh Liu, Masahito Suiko, Yoichi Sakakibara

Recovery of lignin peroxidase from submerged liquid fermentation of Amauroderma rugosum (Blume & T. Nees) Torrend using polyethylene glycol/salt aqueous two-phase system
Wan Yng Linda Jong, Pau Loke Show, Tau Chuan Ling, Yee Shin Tan

Highlights

・ATPS is used to extract and purify lignin peroxidase (LiP) from the submerged fermentation of Amauroderma rugosum.
・The fermentation medium that produce the highest LiP enzyme contains PDB, 0.5% yeast and 1.0% saw dust.
・The addition of NaCl inhibits the LiP enzyme activity by altering the surface properties of the protein.

Isolation and quantification of antimalarial N-alkylamides from flower-head derived in vitro callus cultures of Spilanthes paniculata
Radhika Rajendran, Balaji Sitthu Narashimman, Vishal Trivedi, Rakhi Chaturvedi

Highlights

・Reproducible method for establishment of in-vitro cell cultures from flower heads.
・Controlled, condensed growth and biosynthetic cycle of the cell cultures.
・Pioneer work of chemical analysis of cell culture extract.
・Synchronized accumulation of two powerful N-alkylamides: Spilanthol and UDA.
・One and only report on UDA from plant source.

High glucose enhances cAMP level and extracellular signal-regulated kinase phosphorylation in Chinese hamster ovary cell: Usage of Br-cAMP in foreign protein β-galactosidase expression
Hsiao-Hsien Lin, Tsung-Yih Lee, Ting-Wei Liu, Ching-Ping Tseng

Hypoxia-responsive transgene expression system using RTP801 promoter and synthetic transactivator fused with oxygen-dependent degradation domain
Akihiko Ono, Akira Ito, Tomonaga Sato, Masaki Yamaguchi, Taiga Suzuki, Yoshinori Kawabe, Masamichi Kamihira

Engineering a growth sensor to select intracellular antibodies in the cytosol of mammalian cells
Thuy Duong Nguyen, Hitoshi Takasuka, Yoshihiro Kaku, Satoshi Inoue, Teruyuki Nagamune, Masahiro Kawahara