Molecular mechanisms of the yeast adaptive response and tolerance to stresses encountered during ethanol fermentation
Choowong Auesukaree

Inactivation kinetics and conformation change of Hypocrea orientalis β-glucosidase with guanidine hydrochloride
Xin-Qi Xu, Wei Han, Xiao-Bing Wu, Yong Xie, Juan Lin, Qing-Xi Chen

Highlights

・Production of Hypocrea orientalis cellulase with cassava residues.
・Purification and characterization of H. orientalis β-glucosidase.
・Inactivation kinetics of fungal β-glucosidase by GuHCl was first investigated.
・Active site formed by coiled loops to be obviously flexible.
・Substrate presented protective effect on the active site during denaturation.

Scale-up and inhibitory studies on productivity of lipase from Acinetobacter radioresistens PR8
Krishna Kant Gupta, Anshul Nigam, Sharmili Jagtap, Ramadas Krishna

Highlights

・The three nutrient sources have been compared for lipase productivity from Acinetobacter radioresistens PR8.
・Lower lipase productivity on fresh ground nut is attributed to presence of aflatoxin B1 and oxalic acid.
・Lipase activity has been correlated with lipids profiles in production media.

Efficient antibody production in the methylotrophic yeast Ogataea minuta by overexpression of chaperones
Takeshi Suzuki, Satoshi Baba, Minako Ono, Koichi Nonaka, Kimihisa Ichikawa, Masayuki Yabuta, Rie Ito, Yasunori Chiba

Evaluation of Saccharomyces cerevisiae GAS1 with respect to its involvement in tolerance to low pH and salt stress
Akinori Matsushika, Toshihiro Suzuki, Tetsuya Goshima, Tamotsu Hoshino

Analysis of the oligosaccharides in Japanese rice wine, sake, by hydrophilic interaction liquid chromatography–time-of-flight/mass spectrometry
Masafumi Tokuoka, Chihiro Honda, Akira Totsuka, Hitoshi Shindo, Masaru Hosaka

Metabolite profile of koji amazake and its lactic acid fermentation product by Lactobacillus sakei UONUMA
Yoshifumi Oguro, Toshikazu Nishiwaki, Ryota Shinada, Kazuya Kobayashi, Atsushi Kurahashi

Production of bioethanol from Napier grass via simultaneous saccharification and co-fermentation in a modified bioreactor
Yu-Kuo Liu, Wei-Chuan Chen, Yu-Ching Huang, Yu-Kaung Chang, I-Ming Chu, Shen-Long Tsai, Yu-Hong Wei

Highlights

・Aspergillus niger and Trichoderma reesei were used for cellulose hydrolysis; Zymomonas mobilis, for bioethanol production.
・Effect of CMC concentrations on cellulose hydrolysis and bioethanol production was studied.
・Effect of Napier grass pretreatment on bioethanol production was investigated.
・A repeated fed-batch operation strategy was applied to improve bioethanol production.

Starch-fueled microbial fuel cells by two-step and parallel fermentation using Shewanella oneidensis MR-1 and Streptococcus bovis 148
Megumi Uno, Nichanan Phansroy, Yuji Aso, Hitomi Ohara

Conversion of vegetable wastes to organic acids in leaching bed reactor: Performance and bacterial community analysis
Yan Li, Dongliang Hua, Hui Mu, Haipeng Xu, Fuqiang Jin, Xiaodong Zhang

Microbial secretion of lactate-enriched oligomers for efficient conversion into lactide: A biological shortcut to polylactide
Camila Utsunomia, Ken'ichiro Matsumoto, Sakiko Date, Chiaki Hori, Seiichi Taguchi

Effective modification of cell death-inducing intracellular peptides by means of a photo-cleavable peptide array-based screening system
Ikko Kozaki, Kazunori Shimizu, Hiroyuki Honda

Repression of the DCL2 and DCL4 genes in Nicotiana benthamiana plants for the transient expression of recombinant proteins
Kouki Matsuo, Takeshi Matsumura

Efficient production of antibody Fab fragment by transient gene expression in insect cells
Keita Mori, Hirotsugu Hamada, Takafumi Ogawa, Yuki Ohmuro-Matsuyama, Tomohisa Katsuda, Hideki Yamaji

Phenotypic heterogeneity of human retinal pigment epithelial cells in passaged cell populations
Rie Sonoi, Mee-Hae Kim, Kenta Yamada, Masahiro Kino-oka

Compartmentalized microfluidic perfusion system to culture human induced pluripotent stem cell aggregates
Yuki Kondo, Koji Hattori, Shota Tashiro, Eri Nakatani, Ryosuke Yoshimitsu, Taku Satoh, Shinji Sugiura, Toshiyuki Kanamori, Kiyoshi Ohnuma

Establishment of MicroRNA delivery system by PP7 bacteriophage-like particles carrying cell-penetrating peptide
Yanli Sun, Yanhua Sun, Ronglan Zhao

Highlights

・PP7 VLPs tolerated the insertion of TAT peptide with the cell-penetrating ability.
・PP7 VLPs carrying TAT protected pre-microRNA from the degradation of RNase.
・Pre-miRNA packaged by PP7 VLPs was translated into mature miRNA effectively.
・MiR-23b inhibited the migration of hepatoma cell SK-HEP-1 by downregulating CDH17.
・PP7 VLPs with high yield in Escherichia coli were non-cytotoxic and high thermal stability.

Simple and rapid method for isolation and quantitation of polyhydroxyalkanoate by SDS-sonication treatment
Hisashi Arikawa, Shunsuke Sato, Tetsuya Fujiki, Keiji Matsumoto