Identification and characterization of lbpA, an indigoidine biosynthetic gene in the γ-butyrolactone signaling system of Streptomyces lavendulae FRI-5
Ivy Grace Umadhay Pait, Shigeru Kitani, Yohanes Novi Kurniawan, Maeda Asa, Takashi Iwai, Haruo Ikeda, Takuya Nihira

Characterization of the cellulosomal scaffolding protein CbpC from Clostridium cellulovorans 743B
Daichi Nakajima, Toshiyuki Shibata, Reiji Tanaka, Kouichi Kuroda, Mitsuyoshi Ueda, Hideo Miyake

Improved laccase production by Funalia trogii in absorbent fermentation with nutrient carrier
Guanhua Li, Xiaoxue Liu, Lin Yuan

Metabolic pathway analysis of the xylose-metabolizing yeast protoplast fusant ZLYRHZ7
Jingping Ge, Renpeng Du, Gang Song, Yuhuan Zhang, Wenxiang Ping

Production of 3-hydroxypropionic acid via the malonyl-CoA pathway using recombinant fission yeast strains
Akiko Suyama, Yujiro Higuchi, Masahiro Urushihara, Yuka Maeda, Kaoru Takegawa

Enhancement of butanol production by sequential introduction of mutations conferring butanol tolerance and streptomycin resistance
Yukinori Tanaka, Ken Kasahara, Yutaka Hirose, Yu Morimoto, Masumi Izawa, Kozo Ochi

Effects of exogenous isoprenoid diphosphates on in vivo attachment to bacteriochlorophyllide c in the green sulfur photosynthetic bacterium Chlorobaculum tepidum
Yoshitaka Saga, Hayato Yamashita

Investigation of storage time-dependent alterations of enantioselective amino acid profiles in kimchi using liquid chromatography-time of flight mass spectrometry
Moyu Taniguchi, Yutaka Konya, Yosuke Nakano, Eiichiro Fukusaki

Search for compounds contributing to onion-like off-flavor in beer and investigation of the cause of the flavor
Shigekuni Noba, Nana Yako, Minoru Kobayashi, Susumu Masuda, Tetsuya Watanabe

Anaerobic hydrogen production from unhydrolyzed mushroom farm waste by indigenous microbiota
Chiu-Yue Lin, Chyi-How Lay, I-Yuan Sung, Biswarup Sen, Chin-Chao Chen

Highlights

・Mushroom waste has hydrogen production potential using indigenous microbiota.
・Optimal pH of 8.2 for peak hydrogen production rate was obtained by Ratkowsky model.
・Han-Levenspiel model was slight higher than that compared with Andrew model.

Development of an in situ evaluation system for neural cells using extracellular matrix-modeled gel culture
Takayuki Nagai, Yasuhiro Ikegami, Hideyuki Mizumachi, Nana Shirakigawa, Hiroyuki Ijima

Influence of pH and neutralizing agent on anaerobic succinic acid production by a Corynebacterium crenatum strain
Xiaoju Chen, Xuefeng Wu, Shaotong Jiang, Xingjiang Li

Highlights

・Strategies for pH control in anaerobic fermented phase were optimized to improve succinic acid production by C. crenatum.
・pH 6.8 was the optimal value for anaerobic succinic acid production by C. crenatum.
・Mg(OH)2 used as neutralizing agent for pH control resulting the highest succinic acid concentration.
・Results are helpful for designing a efficient succinic acid production process when Corynebacterium sp. used as strains.

Recombinant and chemo-/bio-orthogonal synthesis of liposomal thrombomodulin and its antithrombotic activity
Lin Wang, Rui Jiang, Yang Liu, Maosheng Cheng, Qingyu Wu, Xue-Long Sun

Highlights

・Liposomal thrombomodulin conjugate was fabricated via direct liposome formation with recombinant TM-lipid.
・Liposomal thrombomodulin conjugate showed potent anticoagulant effect in thrombin-induced thromboembolism mouse model.
・Liposomal thrombomodulin conjugate showed increased circulation time compared to that of recombinant thrombomodulin.

Enhanced production of natural yellow pigments from Monascus purpureus by liquid culture: The relationship between fermentation conditions and mycelial morphology
Jun Lv, Bo-Bo Zhang, Xiao-Dong Liu, Chan Zhang, Lei Chen, Gan-Rong Xu, Peter Chi Keung Cheung

Highlights

・Control of pH and shaking speed in the production of natural Monascus yellow pigments.
・Mycelial morphology of Monascus purpureus was greatly affected by culture conditions.
・The hyphal diameter was correlated with the biosynthesis of Monascus yellow pigments.
・Scale-up production of the Monascus yellow pigments was achieved at 401 U/mL.

Activation of unfolded protein response pathway is important for valproic acid mediated increase in immunoglobulin G productivity in recombinant Chinese hamster ovary cells
Kamal Prashad Segar, Vikas Chandrawanshi, Sarika Mehra

Highlights

・VPA increases qP of Mabs along with increased IgG HC-LC mRNAs in stable CHO cells.
・Transient induction of ER chaperones directly proportional to VPA concentrations.
・p50-ATF6 and XBP1s are key transcription factors to increase qP under VPA.
・HDAC inhibition and increased UPR activity are critical for increase in qP.
・VPA treatment doesn't affect the product quality.

Size- and time-dependent growth properties of human induced pluripotent stem cells in the culture of single aggregate
Suman C. Nath, Masanobu Horie, Eiji Nagamori, Masahiro Kino-oka

Evaluation of method bias for determining bacterial populations in bacterial community analyses
Masayuki Takahashi, Yasuko Kita, Akihiro Mizuno, Nami Goto-Yamamoto